Dynamic culture of human liver equivalents inside a micro-bioreactor for long-term substance testing
نویسندگان
چکیده
Background Current in vitro and animal tests for drug development are failing to emulate the organ complexity of the human body and, therefore, to accurately predict drug toxicity. In this study, we present a self-contained, bioreactor based human in vitro tissue culture test system aiming to support predictive substance testing at relevant throughput. We designed a microcirculation system interconnecting several tissue culture spaces within a PDMS-embedded microfluidic channel circuit. The bioreactor is reproducibly perfused by a peristaltic on-chip micro-pump, providing a near physiologic fluid flow and volume to liquid ratio.
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